IVD Antibody Development Services for Myoglobin Marker

Creative Biolabs is a world-leading service provider of application-specific antibody development. Here, we introduce our in vitro diagnostic (IVD) antibody development and immunoassay development services targeting myoglobin marker. We are confident that our commitment to science and research will enable us to offer you the best products and services.

Myoglobin, a cytoplasmic hemoprotein, is formed independently in oxidative skeletal muscle fibers and cardiac myocytes. It is composed of a single polypeptide chain of 154 amino acids, which has proven to be the backbone of myoglobin containing eight α-helices. Myoglobin combines with oxygen through its heme residue, which is a porphyrin ring:iron ion complex. The polypeptide chain is folded and holds the heme prosthetic group, placing it between two histidine residues, His64 and His93. Then iron ion interacts with six ligands, among which four are offered by the nitrogen atoms of the four pyrroles and share a general plane. The imidazole side chain of His93 offers the fifth ligand, however, the sixth ligand position acts as the binding site for O₂, and other possible ligands such as CO or NO. Myoglobin is extensively regarded as an O₂-storage protein in muscle, enable to liberate O₂ during periods of hypoxia or anoxia. It is also considered to mitigate intracellular O₂ concentration when muscle activity improves, and to promote intracellular O₂ diffusion through offering a parallel path that facilitates simple diffusion of dissolved O₂.

Figure 1. Tertiary protein structure of sperm whale myoglobin. Myoglobin is an iron- and oxygen-binding protein discovered in the muscle tissue of vertebrates and in nearly all mammals. However, in humans, it is just discovered in in the bloodstream after muscle damage. (Wright, T. J. 2015).

Myoglobin Marker of Coronary Artery Disease

Stable CAD enables to induce repetitive reversible myocardial ischaemia, and it is possible that reversibly injured myocardium produces a small number of soluble cytoplasmic proteins. A myoglobin blood test can be used to measure muscle damage. When heart or skeletal muscle is damaged, myoglobin is secreted into the blood and the blood levels of myoglobin increase rapidly which is able to be detected within a few hours following an injury. Sometimes, myoglobin can be used along with troponin as a marker to help measure a heart attack early. Researches have proven that serum concentration of myoglobin above the myocardial infarction (MI) determination threshold is highly connected with a raised risk of six-month mortality, and these results display that myoglobin may be a potential addition to cardiac biomarker panels for early risk-stratification in acute coronary syndromes (ACS).

Figure 2. Sketch map showing the probable influences of myoglobin, eventually regulates physiological functions and diseases. Myoglobin is recognized to be a promising marker for the diagnosis of CAD. (Hendgen-Cotta, U. B. 2010)

Myoglobin Marker of Rhabdomyolysis

Rhabdomyolysis is a clinical syndrome causing by a breakdown of skeletal musculature, with a number of causative agents and clinical performances. A great variety of intracellular constituents, such as myoglobin and creatine kinase (CK) are produced from the injured muscle cells. Recently, a study has demonstrated that a remarkable connection between myoglobin levels in blood and the improved incidence of acute myoglobinuric kidney injury (Mb-AKI) and dialysis dependency in patients with rhabdomyolysis was confirmed. This result suggested that myoglobin may be a potential marker and predictor of suspected rhabdomyolysis and Mb-AKI.

IVD Antibody Development Services Targeting Myoglobin Marker

IVD antibodies have been widely used for disease screening and therapeutic monitoring. As a research partner with years of experience in antibody development and production, Creative Biolabs offers one-stop solutions from antigen design to antibody pair screening to global clients. Besides, we also offer diagnostic immunoassay development services, covering feasibility analysis, assay design, assay protocol establishment, assay optimization, and kit production. Our services can be tailor-designed to adapt to specific project specifications.

If you are interested in our services, please do not hesitate to contact us for more details.

Reference

1. Wright, T. J. (2015). “Myoglobin oxygen affinity in aquatic and terrestrial birds and mammals.” Journal of Experimental Biology 218(14), 2180-2189.
2. Hendgen-Cotta, U. B. (2010). “Unmasking the Janus face of myoglobin in health and disease.” Journal of Experimental Biology 213(16), 2734-2740.
3. Lemos, J. A. (2002). “The Prognostic Value Of Serum Myoglobin in Patients With Non–ST-Segment Elevation Acute Coronary Syndromes.” J Am Coll Cardiol 40(2,238-244).

For Research Use Only.