Human IL1B ELISA Kit, Lot 21AO-263 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P174)

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

<0.15pg/mL

Pre-coated

Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL human IL-1 betastandard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well.

Sample 1: n=16, Mean(pg/ml): 19, Standard deviation: 1.0, CV(%): 5.3
Sample 2: n=16, Mean(pg/ml): 41.2, Standard deviation: 2.6, CV(%): 6.3
Sample 3: n=16, Mean(pg/ml): 116, Standard deviation: 6.5, CV(%): 5.6
Sample 1: n=24, Mean(pg/ml): 27.5, Standard deviation: 1.6, CV(%): 5.8
Sample 2: n=24, Mean(pg/ml): 61.3, Standard deviation: 4.1, CV(%): 6.7
Sample 3: n=24, Mean(pg/ml): 179.1, Standard deviation: 12.7, CV(%): 7.1

Avoid multiple freeze-thaw cycles.

-20 °C,4 °C

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

12 months

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

For Research Use only

Interleukin 1 Beta (IL1b)

IL-1; IL1-BETA; IL1F2; IL-1BETA; IL1beta; il1-b; zgc:111873; IL-1B; IL-1beta; Il-1b; IL1B; IL-1 beta; IL-1b; interleukin 1 beta; interleukin 1; beta; IL1B; il1b; Il1b

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone.

3553

P01584

NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome

A monoclonal antibody from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.