Description
This kit has been developed to isolate highly purified memory CD4+ T cells from human peripheral blood mononuclear cells (PBMCs) by negative selection. Unwanted T cells are depleted by labeling biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoparticles. Isolated cells with up to 75%-85% purity are immediately available for downstream applications such as flow cytometry, culture, or functional assays.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic nanobeads.
Comment
This kit is available for processing 1 x 10^9 cells.
Target
Non-memory CD4+ T cells
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research