Description
This kit has been developed to isolate highly purified human Treg cells ( CD25+CD4+) from human peripheral blood mononuclear cells (PBMCs) by immunomagnetic depletion and the followed positive selection. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or functional assays.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies and magnetic particles; Desired cells are labeled with anti-CD25 antibodies and magnetic particles.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8, CD14, CD15, CD16, CD19, CD36, CD56, CD123, TCRγ/δ and CD235a (glycophorin A); Desired cells are labeled beads targeting CD25.
Method Type
Depletion; positive selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Datasheet