Short Decsription
The kit is designed for in vitro quantitative measurement of Mouse CXCL10 in Cell Culture Supernatant, Plasma, Serum, Tissue Lysate.
Description
For quantitative detection of CXCL10 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Comment
Anti-CXCL10 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCL10 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CXCL10 can be calculated.
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Datasheet