Mouse VTCN1 ELISA Kit, Lot 21AO-1147 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P1058)

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

6 months

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

For Research Use only

V-Set Domain Containing T-Cell Activation Inhibitor 1/Costimulatory molecules B7H4

B7h4; B7s1; B7x; BC032925; B7-H4; B7H4; B7S1; B7X; B7h.5; PRO1291; VCTN1; RGD1311204; V set domain containing T cell activation inhibitor 1 L homeolog; V-set domain containing T cell activation inhibitor 1; V-set domain containing T-cell activation inhibitor 1; V set domain containing T cell activation inhibitor 1; vtcn1.L; VTCN1; vtcn1; Vtcn1

242122

Q7TSP5

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.