PathFinder™ CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit (CAT#: IOK-02P029)

CD27: CD70 [Biotinylation] Inhibitor Screening Assay Kit is used to screen and analyze CD27: CD70 signal transduction inhibitors. The kit uses a convenient 96-well format, biotin-labeled CD70, purified CD27, streptavidin-labeled HRP and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of the detection of biotin-labeled CD70 by streptavidin-HRP. The determination only requires a few simple steps on the microtiter plate. First, the CD27 is coated on a 96-well plate. Next, incubate CD70 with CD27 on the plate. Finally, the plate is treated with streptavidin-HRP, and then HRP substrate is added to generate chemiluminescence, which can be measured using a chemiluminescence reader.

The CD27:CD70[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD27:CD70 signaling. This kit comes in a convenient 96- well format, with biotin-labeled CD70, purified CD27, streptavidin-labeled HRP, and assay buffer for100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD70by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD27 is coated on a 96-well plate. Next, CD70 is incubated with CD27 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

This kit is useful for screening for inhibitors of CD70 binding to CD27.

S152, S152. LPFS2, CD27LG, TNFSF7