PathFinder™ ISRE Reporter Kit (JAK/STAT Signaling Pathway) (CAT#: IOK-LX095)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. The ISRE Reporter kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STAT pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The noninducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.

Available to study immunology signaling pathway, monitor and screen small molecular.

• Monitor IFNa-induced JAK/STAT signaling pathway activity.
• Screen for activators or inhibitors of the JAK/STAT signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.

JAK/STAT Signaling Pathway

Cell Signaling Pathway, Immunology, Onconology