Creative Biolabs is one of the well-recognized experts who are professional in antibody production for a broad range of objectives. With years of experience and advanced technology, we have proudly developed a unique anti-glycan antibody platform to offer a series of anti-glycan antibody related services for worldwide customers. Particularly, our services also involve specific antibody discovery services, which including but not limited to anti-glycan antibody development services, anti-glycan antibody engineering services and anti-glycan antibody detection services.
Carbohydrates, also known as glycans or sugars, are one of the major classes of biomolecules found in various cells such as erythrocytes, immune cells, and microorganisms. And, anti-glycan antibodies are antibodies covering the surface of cells, particularly red blood cells. It has been reported that carbohydrates are critical for numerous biological processes such as cell-cell adhesion, protein folding, protein trafficking, and cell signaling. Moreover, aberrant glycosylation can contribute to a variety of disease states such as cancer and congenital disorders of glycosylation. Antibodies are critical for locating and monitoring the expression of carbohydrates and defining their biological roles. Furthermore, serum anti-glycan antibodies are also potential therapeutic molecules against many cancers.
Fig.1 Cartoon representation and 3D structure of the Fc domain of a typical IgG and its Fc N-glycan.1, 2
Carbohydrate antigens exist in multiple structural configurations, such as polysaccharides, glycoproteins, glycolipids, glycosphingolipids, glycosaminoglycans, and proteoglycans. Given their origins, carbohydrate antigens can be classified as microbial antigens and alloantigens. Microbes produce the former; the latter is expressed by mammalian cells. Microbial carbohydrate antigens include polysaccharides, glycolipids, and glycoproteins. Common alloantigens are blood group carbohydrate antigens synthesized sequentially by gene-specific glycosyltransferases, which catalyze the transfer of sugars from nucleotide-conjugated sugars onto carbohydrate precursors. Recently, multiple carbohydrate antigens have been treated as cancer-related markers for serological diagnosis and treatment of various human cancers.
During the past years, numerous methods have been applied to the production of recombinant antibodies. However, the production of anti-glycan antibodies has been difficult since carbohydrates are self-antigens. Aided by our extensive experience and top technology, Creative Biolabs has successfully established phage display and hybridoma platforms to help global customers discovery more and more antibodies for antibody therapeutics targeting specific molecules. In particular, we have successfully obtained anti-glycan antibodies with reasonably high affinities for reducing mannose residues or tumor-associated antigens.
With the help of experienced scientists and cutting-edge technology, Creative Biolabs is capable of providing a full range of anti-glycan related services to accelerate the development of worldwide customers’ programs. We are happy to share our specialized technology to help our clients with the construction of carbohydrate antigens and the production of anti-glycan antibodies. If you are interested in the services we provide, please feel free to contact us for more information.
References