Creative Biolabs is a contract research organization specialized in offering customized services for the development of antibodies for use in diagnostics, drug discovery, and basic research. Especially, we provide the expertise and personnel to offer high-quality in vitro diagnostic (IVD) antibody development services targeting a wide range of high-value diagnostic biomarkers to our clients. Here, we introduce our IVD antibody development services for creatinine marker.
Creatinine
Creatinine is a byproduct that is derived from muscle metabolism. Creatinine’s formula is C4H7N3O and the molecular weight is 113.1 g/mol. Creatinine is produced from the phosphate of creatine that is a major importance for energy production in muscles. Generally, about 1-2% creatine in the muscles is converted to creatinine every day and creatinine produced at a relatively constant rate by the body. Normally, synthetic creatinine in muscle release into the bloodstream, then almost exclusively filter into the urine by glomerular filtration and is not reabsorbed by the renal tubule includes.
Fig.1 Creatinine-tautomerism-3D-balls.1
Creatinine Marker of Acute Kidney Injury (AKI)
AKI is an abrupt or rapid deficiency of kidney functions. It is a complex clinical syndrome which can influence various biological mechanisms involving immunity, inflammation, apoptosis, and cell cycle. To predict AKI in the early period is difficult, which increases difficulties to develop preventive and therapeutic measures for AKI. Now, more and more evidence reveal that creatinine is a promising indicator to monitor kidney damage or kidney disease.
Under normal circumstances, the creatinine production remains essentially unchanged on a daily basis because of the relatively constant muscle mass. Males produce approximately 150-200 micromols of creatinine per kilogram of body weight per 24 hours while females produce approximately 100-150 micromols of creatinine per kilogram of body weight per 24 hours. However, when there is kidney injury, the kidney function will not work efficiently. The kidneys are not able to filter creatinine, as a result of increased creatinine levels in the blood. Hence, the creatinine clearance (CrCl) may be calculated via measure creatinine levels in blood and urine. And CrCl is associated with glomerular filtration rate (GFR) which is a clinically important indicator of kidney function. Blood creatinine levels may also be solely applied to calculate the GFR. However, the CrCl rate will be exaggerated in the cases of severe renal dysfunction because hypersecretion of creatinine by the proximal tubules will account for a larger portion of the total creatinine secretion. While the usage of ketoacids, cimetidine, and trimethoprim will increase the accuracy of the GFR estimate via reducing creatinine tubular secretion. So, measuring creatinine levels in blood and urine can be used to assess kidney function.
Fig.2 Creatinine generation and clearance dynamics.2
IVD Antibody Development Service Targeting Creatinine Marker
As a leader in the advancing antibody market, Creative Biolabs provides custom IVD antibody development and production services to scientists around the world to support and accelerate their IVD projects. The services we provide include antibody generation, antibody conjugation, antibody pairing, and immunoassay development. our services are fully customized against different targets, including the creatinine marker.
If you are interested in working together or have questions about our services, please contact us or send us an inquiry.
References
For Research Use Only.
