A-431 In Vitro RNA Expression Analysis Assay (Real-time PCR) (CAT#: ITS-1122-YF1348)

A-431

Human

A-431 is a cell line exhibiting epithelial morphology that was isolated from the epidermis of an 85-year-old female patient with epidermoid carcinoma. This cell line is one of a series of cell lines established from solid tumors by D.J. Giard, et al. and can be used in cancer, toxicology, and immuno-oncology research.

A431

Epidermoid Carcinoma

Oncology

In Vitro RNA Expression Analysis Assay (Real-time PCR)

A-431-cell based In Vitro RNA Expression Analysis Assay (Real-time PCR)

Real-time PCR is the most commonly used platform to evaluate mRNA or miRNA expression after conversion of mRNA or miRNA into cDNA. Amplification of a target gene is evaluated by quantifying th fluorescence signal generated using DNA intercalating dyes (SYBER green) or gene-specific probes. This technique can also be used to screen gene mutations, DNA modifications and confirm results obtained from northern blotting or microarray. Additionally, real-time PCR is the most suitable tool for gene expression analysis for RNA samples with low yields compared to hybridization techniques.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.