Cat IFNG ELISA Kit, Lot 21AO-1019 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P930)

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

0.24 ng/mL

100 μL

Pre-coated

-20 °C

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

6 months

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

For Research Use only

Interferon gamma

IFG; IFI; IFN-g; Ifg; IFNG2; IFN-gamma; IFN-G; IFNG; IFNgamma; TCRalpha; INF-G; ifng; interferon gamma; interferon; gamma 1-2; IFNG; Ifng; ifng1-2

Gene Names: IFNG
Protein names: Interferon gamma (IFN-gamma)

493965

P46402

Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy

1.Prepare all reagents, samples and standards.
2.Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C.
3.Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C.
4.Aspirate and wash 3 times.
5.Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C.
6.Aspirate and wash 5 times.
7.Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C.
8.Add 50μL Stop Solution. Read at 450nm immediately.