CellOnco™ HEK293T-Tg(Human BLT1 Receptor) Division-Arrested Cell, Single Clone (CAT#: ITS-0624-HMM600)

Creative Biolabs offers HEK293T-Tg(Human BLT1 Receptor) Division-Arrested Cell which BLT1 receptor stably expressed in HEK293T cells.

HEK293T-Tg(Human BLT1 Receptor) Division-Arrested Cell was engineered to express the receptor human BLT1 (NM_181657). This cell line can be used to study BLT1 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

BLT1 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

BLT1

Leukotriene/Lipoxin

Human

HEK293T

Expression vector containing full-length human LTB4R cDNA (GenBank Accession Number NM_181657) with FLAG tag sequence at N-terminus

NM_181657

Leukotriene B4 (LTB4) is a potent lipid mediator of allergic and inflammatory reactions, as well as a modulator of immune responses. Two types of plasma membrane receptors for LTB4 have been described on human neutrophils. The high-affinity human leukocyte LTB4 receptor, BLT1 (or LTB4R) mediates aggregation, chemotaxis, chemokinesis, and increased adherence to surfaces, whereas the low-affinity receptor mediates degranulation and increased oxidative metabolism. BLT1 mRNA is expressed in leukocytes and to a lesser degree in spleen and thymus. Studies in mice with disrupted BLT1 gene indicate a major role for BLT1 in acute inflammation and immediate hypersensitivity, as well as in leukocyte functions such as chemotaxis and firm adhesion to endothelium in response to LTB4.