CellOnco™ HEK293T-Tg(Human GPR3 Receptor) Division-Arrested Cell, Single Clone (CAT#: ITS-0624-HMM653)

Creative Biolabs offers HEK293T-Tg(Human GPR3 Receptor) Division-Arrested Cell which GPR3 receptor stably expressed in HEK293T cells.

HEK293T-Tg(Human GPR3 Receptor) Division-Arrested Cell was engineered to express the receptor human GPR3 (NM_005281.2). This cell line can be used to study GPR3 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

GPR3 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

GPR3

Orphan

Human

HEK293T

Expression vector containing full-length human GPR3 cDNA (GenBank accession number NM_005281.2) with FLAG tag sequence at N-terminus

NM_005281.2

GPR3 cDNA was first isolate from a rat insulinoma cell line and a human neuroblastoma cDNA library. It expresses in low abundance predominantly in the central nervous system and at low levels in lung and kidney. GPR3 is involved in maintaining meiotic arrest in mammalian oocytes. Oocytes from GPR3 knockout mice resumed meiosis within antral follicles, independently of an increase in luteinizing hormone. In GPR3-null mice, progressive reduction in litter size was observed. Aging GPR3-null mice had severe reduction of fertility, manifested by an increasing number of non-developing early embryos upon spontaneous ovulation and massive amounts of fragmented oocytes after superovulation. Based on the above evidence, it was concluded that GPR3 plays a role in the protection or rescue of oocytes from aging.