CellOnco™ HEK293T-Tg(Human H2 Receptor) Division-Arrested Cell, Single Clone (CAT#: ITS-0624-HMM594)

Creative Biolabs offers HEK293T-Tg(Human H2 Receptor) Division-Arrested Cell which H2 receptor stably expressed in HEK293T cells.

HEK293T-Tg(Human H2 Receptor) Division-Arrested Cell was engineered to express the receptor human H2 (NM_022304.2). This cell line can be used to study H2 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

H2 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

H2

Histamine

Human

HEK293T

Expression vector Application: containing full-length human HRH2 cDNA (GenBank accession number NM_022304.2) with FLAG tag sequence at N-terminus

NM_022304.2

Histamine is one of the most studied biomolecules in medicine and is most notably known for its effects on smooth muscle contraction, vascular permeability and regulation of stomach acid. H2 receptors are positively coupled to adenylate cyclase via Gs. It increases the intracellular Ca2+ concentrations and release Ca2+ from intracellular stores by coupling to Gq. H2 receptors have been found to be located in a variety of tissues, including the brain, gastric cells, and cardiac tissue. Histamine H2 receptors have a potent effect on gastric acid secretion, and the inhibition of this secretory process by H2 receptor antagonists has provided evidence for an important physiological role of histamine in the regulation of gastric secretion. It also regulates gastrointestinal motility and intestinal secretion and is thought to be involved in regulating cell growth and differentiation. It has also been demonstrated to control the relaxation of smooth muscles.