Congo Red Puchtler Kit (CAT#: ITS-1022-ZP43)

The three-reagent kit for staining amyloid characterized by its high ionic strength and pH to enhance the specificity of Congo red dye binding to amyloid clusters. The method developed by Puchtler remains the gold standard for amyloid in tissue sections. Amyloid clusters are birefringent and appear green in polarized light.

The Congo Red Puchtler kit is used to stain amyloid (amorphous clusters) in tissue sections. Despite their differences in protein composition, amyloid deposits are associated with fibrillar beta sheet formation. Congo red dye binds to fibrils and forms a regular pattern of dye molecules in the process. The structural regularity of the dye under polarized light shows the birefringence of light by showing green color. According to Puchtler, the high pH level and ionic strength of the solution allow for higher dye specificity for amyloid.

Tissue Stainning