CT26.WT In Vitro Metaphase Chromosome Analysis Assay (CAT#: ITS-1122-YF1131)

CT26.WT

Mouse

CT26 is an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT. Use CT26.WT mouse fibroblasts with CT26.CL25 as a model for testing immunotherapy protocols and in studies on the host immune response.

CT26

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Metaphase Chromosome Analysis Assay

CT26.WT-cell based In Vitro Metaphase Chromosome Analysis Assay

Metaphase chromosome analysis has become one of the main important tools in cancer genetics. As metaphase chromosomes are highly condensed and coiled, they are more appropriate for banding analysis and to subject for karyotyping.

Genome Alteration Assays

Aberrant or somatic mutations are more commonly found in the DNA of cancer cells compared to normal cells. There is an equilibrium that exists between DNA damage and repair in normal cells. However, in cancer cells these events are disturbed, resulting in mutations and genomic instability. Genomic instability in cancer cells causes chromosomal aberrations, microsatellite instability, aneuploidy and uncontrolled gene amplifications and genetic instability in cancer cells are mainly due to point mutations or chromosomal aberrations such as insertions, deletions and translocation, resulting in mutated proteins.