EL4 In Vitro TRAP Assay (Senescence) (CAT#: ITS-1022-YF2042)

EL4

Mouse

EL4 is a T lymphoblast that was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. This cell line can be used in immunology research.

T Lymphoma

Oncology

In Vitro TRAP Assay (Senescence)

EL4-cell based In Vitro TRAP Assay (Senescence)

The telomerase repeated amplification protocol (TRAP) assay is one of the common PCR-based assays used to detect telomerase activity in cells.

Telomerase Repeated Amplification Protocol Assay

Cellular Senescence in Cancer Assays

Cell senescence is described as an irreversible physiological event of cell growth arrest, which is known to be caused by alterations of telomeres, oxidative stress, DNA damage and some oncogenes. Inhibition of the program of senescence is one of the main features of tumor cells and it has been reported that cancer cells can be induced to undergo senescence by chemo- and radiotherapies and genetic alterations. Telomeres help to prevent uncontrolled cell proliferation and the absence of telomeres results in aneuploidy and cell death.

TRAP Assay