HCT-15 In Vitro Agarose Gel Electrophoresis-based DNA Fragmentation Assay (Apoptosis ) (CAT#: ITS-1122-YF40)

HCT-15

Human

HCT-15 cells were isolated from the large intestine of a male Dukes C colorectal cancer patient and can be used for cancer and toxicology research.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Agarose Gel Electrophoresis-based DNA Fragmentation Assay (Apoptosis )

HCT-15-cell based In Vitro Agarose Gel Electrophoresis-based DNA Fragmentation Assay (Apoptosis )

Laddering of fragmented DNA (oligo-nucleosomal fragments) is detected by agarose gel electrophoresis after staining with DNA intercalating dyes such as ethidium bromide, PI or SYBR green.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

DNA Fragmentation and Damage Assays

DNA Fragmentation Assay