HCT-8 In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis) (CAT#: ITS-1122-YF163)

HCT-8

Human

HCT-8 [HRT-18] cells were isolated from the large intestine of a 67-year-old, male, adenocarcinoma patient. HCT-8 [HRT-18] are used for cancer and toxicology research.

HRT-18

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis)

HCT-8-cell based In Vitro Matrix Metalloproteinase Activity Assay (Angiogenesis)

Matrix metalloproteinases (MMPs) belong to a family of proteases (consist of 24 known human proteases), which can proteolytically degrade ECM protein. Angiogenesis involves the formation of new blood vessels, degradation and remodeling of the ECM to permit endothelial cell migration and invasion into
the neighboring tissue. MMPs have thus been considered in involving remodeling of ECM in angiogenesis. Several MMPs have been identified to enhance angiogenesis by forming promigratory ECM components, responsible for the cleavage of endothelial cell-cell adhesions and release of angiogenic growth factors bound to ECM. MMP activities are modified at transcription level, by endogenous inhibitors and by tissue inhibitors of metalloproteinases. Different assay approaches based on immunochemical and enzyme activities have been introduced by several manufacturers to assess MMP activity at cellular level.

Angiogenesis Monitoring Assays

Local and regional metastasis is a life-threatening condition in cancer, which is hard to control with the available cancer treatment options. In metastasis, cancer cells move away from primary tumors and travel through the blood and lymphatic system and form new tumors on other parts. A vascular
network growth is important for cancer metastasis. Angiogenesis is a process that is known as the formation of new blood vessels with the help of existing blood vessels and angiogenesis has been reported to play a major role in cancer growth and metastasis. Tumors can send chemical signals to nearby normal tissues to stimulate angiogenesis. New blood vessels can feed growing tumors with nutrients and oxygen, allowing cancer cells to spread (metastasis).