Hep 3B In Vitro Agarose Gel Electrophoresis-based DNA Fragmentation Assay (Apoptosis ) (CAT#: ITS-1022-YF2140)

Hep 3B

Human

Hep 3B is a cell line exhibiting epithelial morphology that was isolated from liver tissue derived from an 8-year-old, Black youth with liver cancer. This cell line contains an integrated hepatitis B virus genome.

Hep-3B; Hep3B; Hep 3B2.1-7

Hepatocellular Carcinoma

Oncology

In Vitro Agarose Gel Electrophoresis-based DNA Fragmentation Assay (Apoptosis )

Hep 3B-cell based In Vitro Agarose Gel Electrophoresis-based DNA Fragmentation Assay (Apoptosis )

Laddering of fragmented DNA (oligo-nucleosomal fragments) is detected by agarose gel electrophoresis after staining with DNA intercalating dyes such as ethidium bromide, PI or SYBR green.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

DNA Fragmentation and Damage Assays

DNA Fragmentation Assay