Hs 895.T In Vitro XTT Assay (Cell Viability) (CAT#: ITS-1122-YF2629)

Hs 895.T

Human

This line along with Hs 895.Sk, derived from the same patient, provide tumor and normal counterparts for comparative in vitro studies. This melanoma cell line was isolated from the same patient as Hs 895.Sk, a normal skin fibroblast.

Melanoma

Oncology

In Vitro XTT Assay (Cell Viability)

Hs 895.T-cell based In Vitro XTT Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) can also gener ate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubiliza tion step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins