Human CD40 ELISA Kit, Lot 21AO-364 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P275)

96-well Strip Microplate pre-coated with DNA probes.
DNA Binding Buffer.
Positive Control Sample.
Specific Competitor DNA probe.
Non-specific Competitor DNA probe.
Assay Reagent.
DTT
Wash Buffer.
Primary Antibody.
HRP-conjugated Secondary Antibody.
TMB One-Step Substrate Reagent.
Stop Solution.

Distilled or deionized water.
100 mL and 1 liter graduated cylinders.
Tubes to prepare sample dilutions Absorbent paper.
Precision pipettes to deliver 2 μL to 1 mL volumes.
Adjustable 1-25 mL pipettes for reagent preparation.
Benchtop rocker or shaker.
Microplate reader capable of measuring absorbance at 450 nm

100 μL

Pre-coated

-20 °C

Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.

6 months

Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge.

For Research Use only

p50

Bp50; CDW40; TNFRSF5; p50; AI326936; GP39; HIGM1; IGM; IMD3; T-BAM; TRAP; Tnfrsf5; TNFSF5; CD40 molecule; CD40 antigen; CD40; Cd40

958

P25942

NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.