Material not included
Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl.
Assay Procedure
Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CD80 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CD80 standard solution and each sample be measured in duplicate.
Assay Precision
Sample 1: n=16, Mean(ng/ml): 0.28, Standard deviation: 0.015, CV(%): 5.2
Sample 2: n=16, Mean(ng/ml): 1.56, Standard deviation: 0.111, CV(%): 7.1
Sample 3: n=16, Mean(ng/ml): 2.31, Standard deviation: 0.113, CV(%): 4.9
Sample 1: n=24, Mean(ng/ml): 0.25, Standard deviation: 0.016, CV(%): 6.2
Sample 2: n=24, Mean(ng/ml): 1.72, Standard deviation: 0.138, CV(%): 8.0
Sample 3: n=24, Mean(ng/ml): 2.48, Standard deviation: 0.134, CV(%): 5.4
Handling Advice
Avoid multiple freeze-thaw cycles.
Storage Comment
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Note
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Restrictions
For Research Use only