Short Decsription
ELISPOT detection is based on sandwich immunoenzyme technology. Cytokines or soluble molecules secreted by cells are captured by coating antibodies to avoid diffusion in the supernatant, protease degradation or binding to soluble membrane receptors. After the cells are removed, the captured cytokines are displayed by the tracer antibody and the appropriate conjugate.
Description
The purpose of ELISPOT analysis is to enumerate cytokine producing cells in a single cell suspension. The advantage of this method is that it requires minimal in vitro manipulations, making the analysis of cytokine production as close to in vivo conditions as possible, and in a highly specific manner. The technology aims to determine the frequency of cytokine production under a given stimulus, and to track this frequency in therapeutic and/or pathological conditions. The ELISPOT test is an ideal tool for TH1/TH2 response, vaccine development, viral infection monitoring and treatment, oncology, infectious diseases, autoimmune diseases and transplantation.
Applications
ELISpot
Molecular Mechanism of Action
After cell stimulation, locally produced cytokines are captured by a specific monoclonal antibody. After cell lysis, trapped cytokine molecules are revealed by a secondary biotinylated detection antibody, which is in turn recognised by streptavidin conjugated to alkaline phosphatase. PVDF-bottomed-well plates are then incubated with BCIP/NBT substrate. Colored "purple" spots indicate cytokine production by individual cells.
Target
Fas Ligand
Reactivity
Human
Detection Method
Sandwich
Method Type
Sandwich (qualitative)
Sample Type
cell suspension
Specificity
Recognizes natural human Fas Ligand.
Research Areas
Cell Biology;Immunology

For Research Use Only | Not For Clinical Use

Online Inquiry
Related Products
Copyright © 2024 Creative Biolabs. All Rights Reserved.