Short Decsription
The kit is designed for in vitro quantitative measurement of Human MICA in Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Lysate.
Description
Sandwich ELISA for Quantitative Detection of Antigen
Application Notes
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human MICA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue lysates or serum to each empty well.
ELISA Dilution: 62.5pg/mL-4000pg/mL.
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Lysate, Cell Culture Supernatant, Serum, Tissue Lysate
Datasheet