Human TNFRSF4 ELISA Kit, Lot 21AO-588 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P499)

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

<10pg/mL

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human TNFRSF4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well.

Sample 1: n=16, Mean(pg/ml): 236, Standard deviation: 11.1, CV(%): 4.7
Sample 2: n=16, Mean(pg/ml): 759, Standard deviation: 42.5, CV(%): 5.6
Sample 3: n=16, Mean(pg/ml): 1245, Standard deviation: 79.68, CV(%): 6.4,
Sample 1: n=24, Mean(pg/ml): 250, Standard deviation: 13.25, CV(%): 5.3
Sample 2: n=24, Mean(pg/ml): 838, Standard deviation: 50.28, CV(%): 6
Sample 3: n=24, Mean(pg/ml): 1335, Standard deviation: 96.12, CV(%): 7.2

Avoid multiple freeze-thaw cycles.

-20 °C,4 °C

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

12 months

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

For Research Use only

Tumor necrosis factor receptor superfamily member 4

TNFSF4; OX40L; TNFRSF4; CD134L; CD252; GP34; OX-40L; OX4OL; TXGP1; Ath-1; Ath1; Ox40l; Txgp1l; gp34; TNF superfamily member 4; tumor necrosis factor (ligand) superfamily; member 4; TNFSF4; Tnfsf4

Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily.

7293

P43489

Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints

A monoclonal antibody from mouse specific for TNFRSF4 has been precoated onto 96-well plates. Standards(NSO, L29-A216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.