Description
This kit has been developed to isolate untouched and highly purified naïve CD4+ T cells (CD3+/CD4+/CD62L+/CD44low) from mouse spleen by negative selection. Non-CD4+ Naïve T cells are depleted by labeling biotin conjugated antibody cocktail mixture followed by incubation with magnetic streptavidin nanoparticles. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, immune status monitoring, or functional assays.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles.
Comment
This kit is available for processing 1 x 10^9 cells.
Target
Non-naïve CD4+ T cells
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse spleen
Datasheet