Description
This kit has been developed to isolate highly purified mouse T cells from mouse spleen by negative isolation in 30 minutes. The microbubble system is exceptionally gentle on cells, helping to maintain the health and physiology of monocytes during enrichment. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cell-based experiments.
Features
No magnet, no column, no harsh chemicals
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and depleted.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with antibodies targeting B220 (CD45R), CD8a, CD11b, CD11c, CD24, CD49b, Gr-1, TCR‑γδ and TER-119.
Method Type
Negative selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse spleen
Datasheet