Description
This kit has been developed to isolate untouched and highly purified CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by positive selection. Isolated cells with up to 95% purity are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles
Comment
This kit is available for processing 1 x 10^9 cells
Target
Phosphatidylserine on the outer leaflet of the cell membrane of apoptotic cells using Annexin V
Method Type
Negative Selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Cord Blood, Leukapheresis, Lung, Lymph Node, Other, Spleen