Components
Coating antibodies; Biotinylated detection antibodies; fluorescent-labeled conjugate; Blocking stock solution; Dilution buffer R; Spot enhancer; Tween-20; PVDF FluoroSpot plates with lids and cover slips; Manual;
Assay Procedure
See manual for details.
Assay Measures
FluoroSpot is used to simultaneously detect two cytokines produced by secretory T cells. The method is based on antibodies labeled with different fluorophores, allowing the detection of T cells that produce two pre-selected cytokines. This method is particularly useful for the analysis of T cell subsets with different cytokine profiles.
Storage
Depends on the product components.
Storage Comment
Store protected from light.
Restrictions
For research use only. Not for use in diagnostic procedures.