Description
This kit has been developed to isolate hf hematopoietic non-lineage committed cells (lineage negative) from mouse bone marrow by immunomagnetic positive selection. Lineage committed cells (lineage positive) include T cells, B cells, monocytes/macrophages, granulocytes, and erythrocytes are depleted by labeling biotin conjugated antibody cocktail followed by incubation with magnetic nanoparticles. Isolated cells highly expressed CD117 or Sca-1 are immediately available for downstream applications such as flow cytometry, counting, or other applications.
Applications
Cell Isolation
Application Notes
Unwanted cells are removed by immunomagnetic depletion.
Comment
This kit is available for processing 1 x 10^9 cells.
Molecular Mechanism of Action
Unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting s CD5, CD45R (B220), CD11b, Anti-Gr-1(Ly-6G/C), 7-4 and Ter-119.
Target
Lineage positive Cell
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Mouse bone marrow
Datasheet