PharmaOnco™ CellSort Two-Step Human Memory B Cell Selection Kit (CAT#: ITS-1222-YT4)

This kit has been developed to isolate highly purified memory B cells from human peripheral blood mononuclear cells (PBMC). The isolation of memory B cells is performed in a two-step procedure, negative selection and positive selection. Isolated cells are available for downstream applications such as flow cytometry, culture, or cell-based experiments.

Cell Isolation

First step (negative selection): Unwanted cells are labeled with antibodies and deplete; Next step (positive selection): Desired cells are selected with CD27 MicroBeads.

This kit is available for processing 2 x 10^9 cells.

Step 1: Non B cells; Step 2: CD27+ B cells.

Negative selection & positive selection

Cells

Immunology, Stem Cell Biology, Cell Therapy Research

Human PBMC