Description
This kit has been developed to isolate untouched and highly purified mouse Treg cells ( CD25+CD4+) from mouse spleen by immunomagnetic negative selection and the followed positive selection. Isolated cells with up to 84-94% purity are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Applications
Cell Isolation
Application Notes
Unwanted cells are labeled with antibodies and magnetic particles; Desired cells are labeled with antibodies and magnetic particles.
Comment
This kit is available for processing 1 x 10^9 cells.
Method Type
Negative selection; positive selection
Research Areas
Immunology, Stem Cell Biology, Cell Therapy Research
Sample Source
Other, Spleen