PharmaOnco™ Jurkat-TCR KO//B2M KO Stable Cell Line, Single Clone (CAT#: ITS-0822-HMM209)

Creative Biolabs offers Jurkat-TCR KO//B2M KO stable cell line which the TCR and B2M gene were knocked out in Jurkat cells.

In this Jurkat-TCR KO//B2M KO stable cell line, both the T cell receptor (TCR) and beta-2 microglobulin (B2M) genes have been knocked out using gene editing techniques. The TCR is the receptor on T cells that recognizes antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. By knocking out the TCR gene, the cells are unable to respond to antigen stimulation through this pathway. B2M is a protein that is required for the proper expression of MHC class I molecules on the cell surface. Knocking out B2M disrupts the MHC class I pathway, preventing the cells from presenting antigen to cytotoxic T cells. This double knockout stable cell line can be used in research to study T cell signaling pathways, immune responses, and the interactions between T cells and antigen-presenting cells.

Loss of gene function;
Stability and Specificity;
Reliability and reproducibility;

Functional assay

TCR//B2M

Human

Jurkat

The TCR gene, or T-cell receptor gene, is a gene responsible for encoding the protein receptors on the surface of T cells. T cells are a type of white blood cell that play a central role in the immune system, recognizing and responding to foreign antigens such as viruses, bacteria, and cancer cells. There are two main types of TCR genes, alpha and beta, which combine to form the T-cell receptor complex. These receptors are highly diverse and can recognize a wide variety of antigens, allowing T cells to mount specific immune responses to different threats. Mutations or dysregulation of the TCR gene can lead to immune system dysfunction, resulting in conditions such as autoimmune diseases, immunodeficiency disorders, and cancer. Studying the TCR gene and its role in immune function is important for understanding and developing treatments for these conditions.
The B2M gene encodes the Beta-2 microglobulin protein, which plays a role in the immune system by forming part of the major histocompatibility complex (MHC) class I molecule. This molecule is important for presenting antigens to T cells, allowing the immune system to recognize and destroy infected or abnormal cells. Mutations in the B2M gene can lead to various immune system disorders, including autoimmune diseases and immunodeficiency. Additionally, elevated levels of Beta-2 microglobulin in the blood have been associated with certain types of cancer, such as multiple myeloma and lymphoma.