J82 In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress) (CAT#: ITS-1022-YF3016)

J82

Human

Bladder Cancer

Oncology

In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

J82-cell based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

It's a commonly used simple antioxidant assay. In the DPPH assays, DPPH free radical (DPPH) accepts protons from compounds with antioxidant properties to form yellow colored stable DPPH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Antioxidant Assay