Assay Name
In Vitro H2O2 Assay (Oxidative Stress)
Short Description
Jurkat-Clone E6-1-cell based In Vitro H2O2 Assay (Oxidative Stress)
Assay Description
Some products of chemical reactions/modifications (e.g., H2O2 and GSH) have been considered as ideal indicators of cellular oxidative stress. H2O2 concentration increases during oxidative stress at cellular level.
Assay Type
Oxidative Stress Assays
Assay Type Details
Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.
Assay Subtype
Markers Thiobarbituric Acid Reactive Substances