Jurkat In Vitro Boyden Chamber Assay (Invasion) (CAT#: ITS-1122-YF5698)

Jurkat

Human

The Jurkat cell line (originally called JM) was established in the mid-1970s from the peripheral blood of a 14-year-old boy with T cell leukemia. Different derivatives of the Jurkat cell line have been mutated to lack certain genes.

Acute Lymphoblastic Leukemia; ALL

Oncology

In Vitro Boyden Chamber Assay (Invasion)

Jurkat-cell based In Vitro Boyden Chamber Assay (Invasion)

The Boyden chamber assay needs a microporous membrane that divides a culture well into two compartments. In the upper compartment, cancer cells are cultured in serum-free medium and the lower compartment contains a chemoattractant. Serum is normally used as the chemoattractant and migrated cells through the membrane can be stained and visualized. Coating the microporous membrane with type 1 collagen or matrigel mimics the ECM membrane, which can be used to study invasion. Invasive cancer cells destruct the microporous membrane and migrate to the bottom of the wells through the membrane. By comparing cell migration through coated and non-coated microporous membranes, the invasive index can easily be determined.

Boyden Chamber Assay (Invasion)

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two dif ferent processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Invasion Assay

Cell Invasion Assay