K562 In Vitro Calcium Concentration-based Apoptosis Assay (CAT#: ITS-1022-YF148)

K562

Human

K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes.

K-562

Chronic Myeloid Leukemia; CML

Oncology

In Vitro Calcium Concentration-based Apoptosis Assay

K562-cell based In Vitro Calcium Concentration-based Apoptosis Assay

The endoplasmic reticulum (ER) plays an important role in the storage and maintenance of Calcium. Release of stored Calcium from the ER and uptake by the mitochondria are activated in apoptosis. Measurement of cytosolic Calcium concentrations as an apoptotic signal has been reported in studies.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Measurement of Cytosolic Ca2+ Concentration Assay