Mouse CD74 ELISA Kit, Lot 21AO-1225 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P1136)

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

6 months

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

For Research Use only

Cluster of Differentiation 74

DHLAG; HLADG; II; Ia-GAMMA; CLIP; Ii; INVG34; Iclp-1; iclp1; wu:fb11f09; wu:fb12a01; wu:fb13d06; wu:fk94e07; CD74 molecule; CD74 antigen (invariant polypeptide of major histocompatibility complex; class II antigen-associated); CD74 molecule; major histocompatibility complex; class II invariant chain a; CD74; Cd74; cd74a

CD74, HLA class II histocompatibility antigen gamma chain, CLIP, DHLAG, HLADG, Ii, II, MHC HLA-DR gamma chain, p33, Ia-gamma, INVG34, CD74 antigen, HLA-DR antigens-associated invariant chain, HLA-DR-gamma, Ia antigen-associated invariant chain, Ia-associated invariant chain

16149

P04441

Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.