Mouse IL1B ELISA Kit, Lot 21AO-257 [Cancer Immune Checkpoint Assay Kit] (CAT#: IOK-05-P168)

1. One 96-well plate pre-coated with anti-mouse IL-1beta antibody 2. Standard: 0.5ml (135pg /mL) 3. Standard diluent buffer: 1.5 ml 4. Wash buffer (30x): 20 ml.

1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Pre-coated

The kit should not be used beyond the expiration date on the kit label.
Do not mix or substitute reagents with those from other lots or sources.
If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

For Research Use only

Interleukin 1 Beta (IL1b)

IL-1; IL1-BETA; IL1F2; IL-1BETA; IL1beta; il1-b; zgc:111873; IL-1B; IL-1beta; Il-1b; IL1B; IL-1 beta; IL-1b; interleukin 1 beta; interleukin 1; beta; IL1B; il1b; Il1b

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. It can reset glucose homeostasis at central levels. It cause oligodendrocyte death in coculture with astrocytes and microglia, and also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes

16176

P10749

IL-1 signaling pathway, NF-κB signaling pathway, MAPK signaling pathway, JAK-STAT signaling pathway, TLR signaling pathway, NLR signaling pathway, Inflammasome activation pathway, Cytokine-cytokine receptor interaction, Apoptosis pathway, Pyroptosis pathway, Immune response modulation, Cell proliferation and differentiation, Angiogenesis pathway, Chemokine signaling pathway.