NCI-H23 In Vitro RNA Expression Analysis Assay (DNA Microarray) (CAT#: ITS-0123-YF1557)

NCI-H23

Human

NCI-H23 [H23] is an epithelial-like cell that was isolated from the lung of a Black, 51-year-old, male patient with adenocarcinoma. This cell line was deposited by AF Gazdar and JD Minna and can be used in toxicology and cancer research.

H23

Non Small Cell Lung Cancer

Oncology

In Vitro RNA Expression Analysis Assay (DNA Microarray)

DNA microarray is another technique that enables the analysis of gene expression. Fluorescent labeled cDNA (with cyanines) synthesized from a test sample is hybridized to target probes with complementary sequences attached on microarray chips and strongly bound complexes are screened after scanning optically.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.