Short Decsription
This product is a ready-to-use IHC kit for staining of DSG1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
Description
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein that is highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers. DSG1 mediates intercellular adhesion and have a crucial role in maintaining epidermal integrity and barrier function. It is also involved in epithelial cell differentiation. Mutations in the DSG1 gene can cause the autosomal dominant disorder striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies and metabolic wasting (SAM syndrome).
Features
Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Analytical Method
Immunohistochemistry
Datasheet