Short Decsription
This product is a ready-to-use IHC kit for staining of m6A (N6-methyladenosine). The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
Description
m6A (N6-methyladenosine) is the most abundant internal modification in mammalian mRNA. This modification is installed by the m6A methyltransferases or termed "writers"such as METTL3 and METTL14, and can be reversed by demethylases that serve as "erasers" such as FTO and ALKBH5. The stability of m6A-modified mRNA is regulated by m6A reader protein YTHDFs, which recognizes m6A and reduces the stability of target transcripts. m6A modification and its regulatory proteins play critical roles in cancer pathogenesis and progression. m6A modification is also invovled in viruses life cycles, suggesting that drugs targets to m6A pathway could be used for antiviral thereapy.
Features
Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Reactivity
Human, Mouse, Rat, N6-methyladenosine
Analytical Method
Immunohistochemistry
Datasheet