Short Decsription
This product is a ready-to-use IHC kit for staining of PTEN. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
Description
PTEN(Phosphatase and tensin homolog) is also named as MMAC1, TEP1 and it modulates cell cycle progression and cell survival (blockage) through down-regulating the positive cell cycle-regulator (cycle D1) by its phosphatase activity and up-regulating the negative cell cycle regulator p21 (by its lipid phosphatase activity). PTEN is an important tumor suppressor whose inactivation frequency in cancers is ranked second after p53 tumor suppressor inactivation. PTEN is a 403-residue protein structured in an amino (N)-terminal phosphatase domain and a carboxy (C)-terminal C2 domain that binds phospholipid membranes. There are some report showing that SUMOylation appears to be a positive regulator in controlling PTEN membrane association, whereas phosphorylation is a negative regulator that may neutralize SUMOylation through intramolecular electrostatic interactions.
Features
Ready to use
High Sensitivity Streptavidin (HSS) increases detection sensitivity
Suitable for tissue sections & cytospin preparations
Pre-diluted reagents minimize hands-on time
Optimizes mouse, rabbit, rat, goat, or sheep IgG primary antibody staining
Reactivity
Human, Mouse, Rat
Analytical Method
Immunohistochemistry
Datasheet