PathFinder™ CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit (CAT#: IOK-02P030)

CD28:B7-1 [Biotinylation] Inhibitor Screening and Analysis Kit is designed to screen and analyze CD28:B7-1 signal transduction inhibitors. The key to this kit is the high sensitivity of the detection of biotin-labeled B7-1 through streptavidin-HRP. The determination only requires a few simple steps on the microtiter plate. First, the CD28 is coated on a 96-well plate. Next, B7-1 was incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP, and then HRP substrate is added to generate chemiluminescence, which can be measured using a chemiluminescence reader.

The CD28:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD28:B7-1 signaling. The key to this kit is the high sensitivity of detection of biotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD28 is coated on a 96-well plate. Next, B7-1 is incubated with CD28 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

This kit is useful for screening for inhibitors of B7-1 binding to CD28.

CD80, B7.1