PathFinder™ Human IDO1 Fluorogenic Inhibitor Screening Assay Kit (CAT#: IOK-LX016)

96 reactions

IDO1 His-Tag;
IDO1 Fluorogenic Reaction Solution;
IDO1 Assay Buffer;
Fluorescence Solution;
Black 96 Well Assay-Plate;
Plate sealing film;

L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

96-well plate

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Gel Packs

In the assay, the inhibitor and enzyme are added to a sample containing L-Trp substrate. After a 1 hourincubation at room temperature, the fluorescence solution is added and incubated at 37°C for four hours. Activity is measured by reading sample fluorescence at l=510 nm following excitation of the reaction product at l=400 nm.