PathFinder™ MLL1 SAM-Screener™ Assay Kit (CAT#: IOK-LX068)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe* that binds to the SAM binding pocket in MLL1. Binding of the small molecule probe to MLL1 induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The MLL1 SAM-Screener™ Assay is robust (Z'>0.6) and exhibits a greater than 100 mP shift over a range of 0-500 nM MLL1. The assay is suitable for high-throughput screening in the provided 384-well plate.

Available to monitor and screen small molecular inhibitors for drug discovery and HTS applications, as well as enzyme kinetics study.

This kit is useful for screening small molecular inhibitors for drug discovery and HTS applications.

Mixed-Lineage Leukemia

Immunology, Onconology