PathFinder™ Mouse CTLA4:B7-1 TR-FRET Assay (CAT#: IOK-02P067)

Mouse CTLA4: B7-1 TR-FRET assay aims to measure the inhibitory effect of the homogeneous 384 response format on the binding of mouse CTLA4 to mouse B7-1. This FRET-based assay does not require time-consuming washing steps, so it is particularly suitable for high-throughput screening applications. The measurement process is simple and straightforward. The samples containing biotinylated mCTLA4, mB7-1 protein, anti-His Tb donor, dye-labeled acceptor and inhibitor were incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

The mouse CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of mouse CTLA4 binding to mouse B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated mCTLA4, mB7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

CD152, cluster of differentiation 152, B7