PathFinder™ Myeloperoxidase Inhibitor Screening Assay Kit (CAT#: IOK-LX076)

Creative Biolabs offers several bioanalysis and inhibitor screening assay kits for customers, which will help researchers to accelarate the studies in immunology and onconology areas. MPO Inhibitor Screening Assay Kit provides convenient fluorescence-based method for identifying novel inhibitors of the chlorination and peroxidation activities of myeloperoxidase (MPO). The chlorination assay uses the non-fluorescence APF. APF is selectively cleaved by hypochlorite (-OCl) to yield free fluorescein. which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 480-495 and 515-525 nm, respectively. The peroxidation assay is based on the two-electron oxidation of native MPO to MPO-I by hydrogen peroxide, followed by two successive one-electron reductions back to MPO by ADHP with an MPO-II intermediate. The reaction between hydrogen peroxide and ADHP yields resorufin, which can be easily quantified at excitation and emission wavelengths of 530-540 and 585-595 nm, respectively.

Available to monitor and screen small molecular inhibitors for drug discovery and HTS applications, as well as enzyme kinetics study.

This kit is useful for screening small molecular inhibitors for drug discovery and HTS applications.

Myeloperoxidase; MPO

Immunology, Onconology